-
[18/07/09]
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[18/07/09]
TOPcloner™ TA-Blunt core Kit
| No | Product Name | Cat.# | Conc | Size | Price(￦) | Add |
|---|---|---|---|---|---|---|
| 1 | TOPcloner™ TA-Blunt core Kit | EZ017S | - | 20 rxns | ￦ 200,000 |
|
| 2 | TOPcloner™ TA-Blunt core Kit | EZ017M | - | 40 rxns | ￦ 360,000 |
|
Product description
TOPcloner™
TA-Blunt core kit are suitable for cloning at room temperature in 5 min with
both Taq polymerase-amplified PCR products (A tail overhangs) and Pfu
polymerase-amplified PCR products (blunt ends). The vector provided in this kit
contains topoisomerase covalently coupled to its ends, which allows efficient
cloning of any DNA fragments to the vector without using a regular ligase.
Characteristics
- Short reaction time
(5 min)
- Highly efficient
cloning
- Direct use of PCR
products
Applications
- Fast and efficient
cloning of both A-tailed and blunt ended PCR products
Supplied
with
- pTOP TA-Blunt V2
- 6X TOPcloner™ Buffer
- Sterile water
Quality
control
- Cloning efficiency:
99%
Standard
reaction conditions
|
6X
TOPcloner™ Buffer |
1 μl |
|
pTOP TA-Blunt V2 (10 ng/μl) |
1 μl |
|
PCR
product |
1~4 μl |
|
Sterile
water |
up to 6 μl |
→ Incubate at room temperature(25℃) for 5 min
→ Transformation
● Enzynomics_TOPcloner™ Cloning kit_manual_kor
● Enzynomics_EZ017_TOPcloner™ TA-Blunt core kit_manual_simple(eng)
● Material Safety Data Sheet
- Enzynomics_MSDS_EZ017_TOPcloner™ TA-Blunt core Kit_E
● Vector Information
