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BL21(DE3) Chemically Competent E.coli

No Product Name Cat.# Conc Size Price(₩) Add
1 BL21 (DE3) Chemically Competent E.coli
(100 μl X11 tubes)
CP110 - 1.1 ml ₩ 60,000 추가
2 BL21(DE3) Chemically Competent E.coli
(100 μl X21 tubes)
CP111 - 2.1 ml ₩ 100,000 추가
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Product description

BL21(DE3) competent cell are expression hosts for protein production using the T7 promoter. The (DE3) designation indicates that the host is a lysogen of λ(DE3), and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are required for protein expression from target genes cloned in T7 expression vectors. BL21(DE3) is the most widely used host for protein expression because it is deficient in the Lon and OmpT proteases.

 

Characteristics

- Strain : BL21(DE3)

- E. coli B strain with DE3, a λ prophage carrying the T7 RNA polymerase gene and lacIq

- Genotype : F– dcm ompT hsdS (rB- mB-) gal λ(DE3)

- Prepared using an optimized chemical method

 

Applications

- T7 promoter protein expression

- Whole genome sequence

 

Quality control

- Transformation efficiency : ≥1.6 × 106 cfu/μg of pUC19

 

Cautions

- Transformation should be started immediately after the cells have thawed on ice.

- Do not use a pipette when thawing competent cells or mixing DNA samples with cells.

- Do not freeze competent cells once thawed

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