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INT (Integrase)

No Product Name Cat.# Conc Size Price(₩) Add
1 INT (Integrase) M023S 100 ng/μl 10 μg 문의 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
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Caution

* 본사에 문의 후 구매하시기 바랍니다.


Product description

The integrase protein of bacteriophage l (INT) catalyzes site-specific recombination between l phage and Escherichia coli genomes.  Int is a tyrosine recombinase that binds to DNA core sites via a C-terminal catalytic domain and to a collection of arm DNA sites, distant from the site of recombination, via its N-terminal domain. The arm sites, in conjunction with accessory DNA-bending proteins, provide a means of regulating the efficiency and directionality of Int-catalyzed recombination (1).

 

Characteristics

Recombination initiates with the pairing of two specific DNA segments by a tetramer of recombinase molecules. A four-wayDNA junction (Holliday junction) is formed by the cleavage, exchange and ligation of one pair of strands, and is resolved to helical DNA products by the exchange of the second pair of strands.

 

Applications

- Site specific recombination

- gene therapy

- construction of transgenic organisms

- gene manipulation

 

Quality control

- Endonuclease-free

- Exonuclease-free

 

Unit Definition

Sold by mass of pure protein as determined by OD280

 

Storage

25 mM Tris-HCl pH 7.4, 0.5 M NaCl, 1 mM DTT, 50% Glycerol

● Material Safety Data Sheet

- Enzynomics_MSDS_M023_INT (Integrase)_E


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