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Taq DNA Ligase

No Product Name Cat.# Conc Size Price(₩) Add
1 Taq DNA Ligase M019S 40 units /μl 2,000 units ₩ 80,000 추가
2 Taq DNA Ligase  M019L 40 units /μl 10,000 units ₩ 320,000 추가
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Product description

A gene encoding Thermus aquaticus DNA ligase is cloned and expressed in E. coli, and the enzyme was purified to homogeneity. This enzyme catalyzes the formation of a covalent bond between the 5’-phosphate and 3’-OH in nicked duplex DNA or at two DNA ends. Thermus DNA Ligase uses NAD+ instead of ATP as a cofactor.

 

Characteristics

- Molecular weight: 73 KDa

- Optimal temperature: 45~65

- Heat inactivation: No.

 

Applications

- Ligation of DNA fragments

- Ligase chain reaction (LCR) for detection of mutations

- Simultaneous mutagenesis of multiple sites

 

Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free

- phosphatase-free

            

Unit Definition

1 unit is defined as the amount of Taq DNA Ligase required to join 50% of 1 μg of the 12-base cohesive ends of Lambda DNA cut with Hind III in 50 μl 1x Taq DNA Ligase Buffer following a 10 minute incubation at 45

 

Storage

10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 200 μg/ml BSA, 50% Glycerol pH 7.4 @ 25, Store at -20.

 

10X Taq DNA Ligase buffer

200 mM Tris-HCl, 250 mM potassium acetate, 100 mM magnesium acetate, 100 mM DTT, 10 mM NAD 1.0% Triton X-100 (pH 7.6 @ 25°C), Store at -70

 

Cautions

- Requires NAD+ as a cofactor

 

Standard reaction conditions             

- Incubate DNA and enzyme in 1X Taq DNA Ligase Buffer at 45 for 15 min.

● Material Safety Data Sheet

- Enzynomics_MSDS_M019_Taq DNA Ligase_E

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