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Thermostable Inorganic Pyrophosphatase

No Product Name Cat.# Conc Size Price(₩) Add
1 Thermostable Inorganic Pyrophosphatase M015S 2 units/μl 250 units ₩ 70,000 추가
2 Thermostable Inorganic Pyrophosphatase M015L 2 units/μl 1,250 units ₩ 280,000 추가
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Product description

Thermostable inorganic pyrophosphatase is a hydrolase specific for inorganic diphosphate. It catalyses the conversion of inorganic pyrophosphate into orthophosphate in reaction mixtures where inorganic pyrophosphate is accumulated followed by DNA synthesis.

 

Characteristics

- Molecular weight: 37 kDa

- Heat inactivation: No

            

Applications

- Enhances PCR or other amplification reactions

- Optimizes PCR through the elimination of pyrophosphate

 

Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free

            

Unit Definition

One unit is the amount of enzyme that will generate 1 μmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 75 in 50 mM Tricine [pH 8.5] , 1 mM MgCl2, 0.32 mM PPi, reaction volume of 0.5 ml).

 

Storage

20 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol pH 8.0 @ 25, Store at -20.

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