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Lambda Exonuclease

No Product Name Cat.# Conc Size Price(₩) Add
1 Lambda Exonuclease M008S 5 units/μl 1,000 units ₩ 75,000 추가
2 Lambda Exonuclease M008L 5 units/μl 5,000 units ₩ 300,000 추가
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Product description

Lambda Exonuclease processively degrades one strand of double-stranded DNA (dsDNA) in the 5'→3' direction. The enzyme has greatly reduced activity on ssDNA and non-phosphorylated DNA. Nick DNA and gap DNA are not usually recognized as starting points. A gene encoding Lambda exonuclease is cloned and expressed in E. coli, and the recombinant Lambda exonuclease is purified to homogeneity.

 

Characteristics

- Molecular weight: 24 kDa

- Reaction temperature: 37

- Heat inactivation: 75 for 10 min

            

Applications

- Generation of ssDNA sequencing template from PCR

  products.

- Restriction mapping.

 

Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

            

Unit Definition

One unit is defined as the amount of enzyme required to produce 10 nmol of acid soluble deoxyribonucleotide from double-stranded substrate in a total reaction volume of 50 μl in 30 min at 37.

 

Storage

25 mM Tris-HCl, (pH 8.0), 50 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol.

 

10X Lambda Exonuclease buffer

670 mM Glycine-KOH (pH 9.4), 25 mM MgCl2, 500 μg/ml BSA.

 

Cautions

- Lambda exonuclease requires Mg2+, and a relatively high pH.

 

Standard reaction conditions

- Radiolabeling at 5’ ends of DNA.

DNA

0.1 μg ~ 1 μg

10X Lambda exonuclase buffer

2 μl

Lambda exonuclase (5 units/μl)

1 μl

Distilled water

Up to 20 μl

→ Incubate at 37 for 30 min.

→ Heat inactivate at 75 for 10 min.

 



Figure 1. Degradation of phosphorylated DNA

Varying amount of restriction digested (pSKMII-Hind III) linear dsDNAs were degraded by Lambda Exonuclease (5 unit)

● Material Safety Data Sheet

- Enzynomics_MSDS_M008_Lambda Exonuclease_E

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