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EZ-Fusion™ Cloning Kit (Dry Type)

No Product Name Cat.# Conc Size Price(₩) Add
1 EZ-Fusion™ Cloning Kit (Dry Type) EZ019S - 8 reactions 문의 추가
2 EZ-Fusion™ Cloning Kit (Dry Type) EZ019M - 16 reactions 문의 추가
3 EZ-Fusion™ Cloning Kit (Dry Type) EZ019L - 96 reactions 문의 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
Total : 장바구니에 담기 바로 구매 하기
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※ 위 제품은 18년 7월 31일까지만 판매되었으며, 개선된 EZ-Fusion™ HT Cloning kit 를 사용하시기 바랍니다.


▶ EZ-Fusion™ Cloning kit principle video

▶ EZ-Fusion™ Cloning kit primer design program


Product description

EZ-Fusion Cloning Kit (Dry Type) is designed for rapid and efficient cloning of PCR-amplified DNA fragments in to any cloning vectors including commercial and customized ones. It is also possible to insert one or more DNA fragments into a cloning vector in a defined orientation. EZ-Fusion Cloning DryMIX allows terminal 10 to 20 base pair overlapping homologous DNA at the ends of linearized vectors (usually by restriction enzymes) and insert DNA fragments (usually PCR-amplified) to precisely recombine to generate cloning products. In addition, the EZ-Fusion Cloning kit (Dry Type) can be used to clone long DNA fragments with high efficiency. EZ-Fusion Cloning Kit (Dry Type) is provided with a convenient lyophilized format of all the reaction components in a single reaction tube, thereby concising reaction set-up and minimizing the variability between reactions.

 

Characteristics

- Ease of use and consistency

- Sub-cloning is unnecessary

- Highly efficient

- Seamless construction

- Flexibility to clone single or multiple fragments

- Can be used to perform site-directed mutagenesis

 

Applications

- PCR cloning

- HTP cloning

- Multiple fragment cloning

- Gene synthesis

- Gene design

- Mutagenesis

- Domain swapping

- Domain modification

 

Quality control

- Cloning efficiency : 99%

 

Kit contents

- EZ-Fusion Cloning DryMIX

- pUC19 Control Vector, linearized (50 ng/µl)

- 2 kb Control Insert (40 ng/µl)

- Sterile water

- DH5α Chemically Competent E. coli



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