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TOPreal™ One-step RT qPCR Kit
(SYBR Green with low ROX)

No Product Name Cat.# Conc Size Price(₩) Add
1 TOPreal™ One-step RT qPCR Kit
(SYBR Green with low ROX)
RT432S - 100 reactions ₩ 130,000 추가
2 TOPreal™ One-step RT qPCR Kit
(SYBR Green with low ROX)
RT432M - 500 reactions ₩ 585,000 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
Total : 장바구니에 담기 바로 구매 하기
plus minus 삭제

 ● Product Selection Guide by qPCR instruments



Product description

TOPreal™ One-step RT qPCR Kit (SYBR Green with low ROX) is a ready-to-use solution optimized for One Step RT qPCR including SYBR Green I and low level of ROX as a reference dye. The easy one-tube setup minimizes the risk of contamination. This kit contains HOT Start PCR polymerase, which prevents extension of nonspecifically annealed primers and primer–dimers, and Reverse Transcriptase, high yield of synthesizing cDNA. The optimized components result in highly sensitive and successful results.

 

Characteristics

- Reduce the contamination risk of RT-qPCR analysis.

- High specificity and sensitivity.

- Normalization of the SYBR Green I: ROX Passive reference dye is included in the master mix.

 

Applications

- Real-time quantification of RNA target

- Gene expression analysis.

 

Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free

- RNase-free

- Inhibitor-free

 

Standard reaction conditions

- RT qPCR mixturea

TOPreal™ One-step RT qPCR Enzyme MIX

1 μl

2X TOPreal™ One-step RT qPCR Reaction MIX

10 μl

Template (total RNA, 10 ng ~ 1 μg/μl)

1 μl

Primers 1 (5~10 pmol/μl)

1 μl

Primers 2 (5~10 pmol/μl)

1 μl

Sterile water (RNase free)

up to 20 μl

aAssemble the reaction mixture on ice

 

- RT qPCR cycle

Reverse Transcription

50

30 min

PCR reaction

Initial denaturation

95

10 min

Denaturation

95

5 sec

Annealing & Elongation

60

30 sec

Number of cycles

30~45 times

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