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TOPreal™ qPCR 2X PreMIX
(TaqMan Probe for multiplex)

No Product Name Cat.# Conc Size Price(₩) Add
1 TOPreal™ qPCR 2X PreMIX
(TaqMan Probe for multiplex)
RT620S 2X 200 reactions ₩ 150,000 추가
2 TOPreal™ qPCR 2X PreMIX
(TaqMan Probe for multiplex)
RT620M 2X 500 reactions ₩ 300,000 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
Total : 장바구니에 담기 바로 구매 하기
plus minus 삭제

 ● Product Selection Guide by qPCR instruments



Product description

TOPreal™ qPCR 2X PreMIX (TaqMan Probe for multiplex) is a ready-to-use PCR mixture containing 2X concentration of DNA polymerase, reaction buffer, and dNTP. The resulting hot-start PCR amplification provides increased sensitivity and specificity. An optimized multiplex PCR master mix designed specifically for Real-time PCR, which makes it possible to detect multiple targets at the same time. This kit provides a level of sensitivity equally or superior than singleplex probe-based real-time PCR. Additionally, this kit minimized the fluorescence signal decrement derived from probe to probe and primer to primer interference.

 

Characteristics

- Multiplexing

- Ease of Use: Premix contains everything needed except for primers, template and probe.

- High specificity: Enhanced specificity by the use of HOT Start PCR polymerase.

- Stability: Enhanced stability allows one year storage at -20

 

Applications

- Pathogen Identification

- High Throughput SNP Genotyping

- Gene Deletion Analysis

- Template Quantitation

- Linkage Analysis

 

Standard reaction conditions

- PCR mixture

TOPreal™ qPCR 2X PreMIX (TaqMan Probe for multiplex)

10 μl

Template DNA

1 μl

Probe (2~4 pmol/μl)

1 μl

Primers 1 (5~10 pmol/μl)

1 μl

Primers 2 (5~10 pmol/μl)

1 μl

Sterile water (RNase free)

up to 20 μl

 

- PCR cycle

Initial denaturationa

95

10~15 min

Denaturation

95

10 sec

Annealing & Elongation

60

60 sec

Number of cycles

35~55 times

aAt least 10 min of initial denaturation time is required to fully activate the chemically modified PCR DNA polymerase

 

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