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TOPscript™ cDNA synthesis Kit (mix type/dT18 plus)

No Product Name Cat.# Conc Size Price(₩) Add
1 TOPscript™ cDNA synthesis Kit
(mix type/dT18 plus)
EZ105S - 100 reaction ₩ 590,000 추가
2 TOPscript™ cDNA synthesis Kit
(mix type/dT18 plus)
EZ105M - 200 reaction ₩ 1,050,000 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
Total : 장바구니에 담기 바로 구매 하기
plus minus 삭제

Product description

TOPscript™ cDNA synthesis Kit (mix type/dT18 plus) is designed for the maximum-efficiency conversion of all types of RNA including mRNA to cDNA. The kit contains RNase inhibitor in TOPscript™ Reverse TranscriptaseR+ to protect RNA degradation and oligo dT (dT18) in 10X TOPscript™ RT reaction MIX (dT18). TOPscript™ Reverse Transcriptase is a highly thermostable version of M-MLV RT (RNase H-), and it can synthesize cDNA at 50~60 with highest fidelity. Thus, it is well suited to synthesize long cDNA (>20 kb) from long and highly secondary-structured RNA.

 

Characteristics

- cDNA synthesis across a broad temperature range

- Synthesis long cDNA

- Include all components required for cDNA synthesis

 

Applications

- First-strand of cDNA synthesis

- Primer extension

 

Supplied with

- TOPscript™ Reverse TranscriptaseR+ (200 U/μl)

- 10X TOPscript™ RT reaction MIX (dT18)

- Sterile water (RNase free)

 

Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free

- RNase-free

- Inhibitor-free

- Satisfactory yield and length of cDNA products

 

Unit definition

One unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid-insoluble materrials using 0.4 mM poly(rA)-oligo(dT) as substrate at 37 in 10 min in a 1X reaction buffer.

 

Storage buffer

50 mM tris-HCl (pH 7.6), 150 mM NaCl, 1mM dTT, 0.1 mM EDTA, 0.1% NP-40, 50% glycerol

 

Standard reaction conditions

10X TOPscript™ RT reaction MIX (dT18)

2 μl

TOPscript™ Reverse TranscriptaseR+ (200 U/μl)

1 μl

a)Template RNA

X μl

Sterile water (RNase free)

up to 20 μl

a)Prepare one of the following RNA template.

    - Total RNA: 1 ng~5 μg

    - Messenger RNA (mRNA): 1 ng~250 ng

    - Specific RNA: 0.01 pg~0.5 μg

→ Incubate at 42~60 for 60 min.

→ Incubate at 95 for 5 min to inactivate the reaction.

 

Note

TOPscript™ Reverse Transcriptase performs optionally over the full range of 42~60. Typically, 50 is a good starting point. For RNAs containing secondary structure and other challenging targets, a synthesis temperature of 60 may be used without loss of performance.

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