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Klenow DNA Polymerase, exo-

No Product Name Cat.# Conc Size Price(₩) Add
1 Klenow DNA Polymerase, exo- KP002S 5 units/ μl 200 units ₩ 62,000 추가
2 Klenow DNA Polymerase, exo- KP002L 5 units/ μl 1,000 units ₩ 248,000 추가
3 Klenow DNA Polymerase, exo- KP002H 50 units/ μl 1,000 units ₩ 248,000 추가
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Description

Klenow DNA polymerase (exo-) is an N-terminal truncation of DNA polymerase I purified from a recombinant source. It has lost the 5´→ 3´ exonuclease activity but retains polymerase activity. D335A and E357A mutations abolish the 3´→ 5´ exonuclease activity.

 

Characteristics

- Isolated from a recombinant source

- Generates probes using random primers

- Dideoxy sequencing

- Moderate strand displacement activity

 

Activity

- 5’→3’ exonuclease: No

- 3’→5’ exonuclease: No

- Strand Displacement: Yes

 

Applications

- Random priming labeling

- DNA sequencing by the Sanger dideoxy method

- Second strand cDNA synthesis

- Second strand synthesis in mutagenesis Protocols

 

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C.

 

Storage Conditions

25 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 @ 25°C, Store at -20°C

 

Heat Inactivation

75°C for 20 min

 

Quality Control

- DNase Activity

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free

 

Supplied with

- 10X Klenow DNA Polymerase buffer

- Sterile water

 

Cautions

- Klenow Fragment (exo-) could not remove non-templated 3´ additions and not suitable for blunt 

  ends generation.

- Klenow Fragment (exo-) is also active in all four EzBuffers in the presence of with dNTP.

- 1 unit/5 μl reaction volume is recommended for sequencing with Klenow Fragment (exo-) by dideoxy 

  method of Sanger et al.

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