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DNA Polymerase I (E. coli )

No Product Name Cat.# Conc Size Price(₩) Add
1 DNA Polymerase I (E. coli ) DP002S 10 units/ μl 500 units ₩ 68,000 추가
2 DNA Polymerase I (E. coli ) DP002L 10 units/ μl 2,500 units ₩ 272,000 추가
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Product description

DNA Polymerase I (E. coli) is over expressed and isolated from E. coli. It has inherent 3´→ 5´ and 5´→ 3´ exonuclease activities other than its polymerase activity. 5´→ 3´ exonuclease activity of DNA Polymerase I allows nick translation by removing nucleotides ahead of the growing DNA chain

 

Characteristics

- Nick translation of DNA

- Second strand cDNA synthesis

 

Activity

- 5’→3’ exonuclease: Yes

- 3’→5’ exonuclease: Yes

- Strand Displacement: No

 

Applications

- Nick translation of DNA to obtain probes with a high specific activity

- Second strand synthesis of cDNA

 

Unit definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C.

 

Reaction Conditions

1X DNA Polymerase I buffer, Incubate at 37°C

 

Storage Conditions

25 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 @ 25°C, Store at -20°C

 

Heat Inactivation

75°C for 20 min

 

Quality Control

- Endonuclease-free

 

Supplied with

- 10X DNA Polymerase I Buffer

- Sterile water

 

Cautions

- DNase I should purchase separately for nick translation reactions.

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