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Bst DNA polymerase (Full length)

No Product Name Cat.# Conc Size Price(₩) Add
1 Bst DNA polymerase (Full length) DP005S 5 units/µl 500 units ₩ 68,000 추가
2 Bst DNA polymerase (Full length) DP005L 5 units/µl 2,500 units ₩ 272,000 추가
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Product description

Full Length Bacillus stearothermophilus Bst DNA Polymerase is highly purified as a recombinant. Other than 5´ → 3´ polymerase activity it has double-strand specific 5´ → 3´ exonuclease activity, but lacks 3´ → 5´ exonuclease activity.

 

Characteristic

- Isolated from a recombinant source

 

Activity

- 5’→3’ exonuclease: Yes (double-strand specific)

- 3’→5’ exonuclease: No

- Strand Displacement: No

 

Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- RNase-free

 

Unit definition 

One unit is defined at the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.

Storage buffer

10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.1% Triton® X-100, pH 7.4 @ 25°C

 

1X Bst DNA Polymerase buffer

20 mM Tris-HCl, 10 mM (NH4)2SO4, 10 mM KCl, 2 mM MgSO4, 0.1% Triton® X-100, pH 8.8 @ 25°C

 

Supplied with

- 10X Bst DNA Polymerase buffer

- Sterile water

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