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T7 DNA Polymerase

No Product Name Cat.# Conc Size Price(₩) Add
1 T7 DNA Polymerase DP003S 10 units/µl 300 units ₩ 68,000 추가
2 T7 DNA Polymerase DP003L 10 units/µl 1,500 units ₩ 272,000 추가
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Description

T7 DNA Polymerase dimer has DNA polymerase and strong 3´→ 5´ exonuclease activities. It catalyzes the replication of T7 phage DNA during infection. The high fidelity and rapid extension rate make it possible to copy long stretches of DNA template.

 

Characteristics

- Isolated from a recombinant source

- Second strand synthesis in site-directed mutagenesis

- Gap filling reaction (no strand displacement)

 

Activity

- 5’→3’ exonuclease: No

- 3’→5’ exonuclease: Yes

- Strand Displacement: No

 

Applications

- Second strand synthesis in site-directed mutagenesis protocols

 

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmoles of dNTP into acid insoluble material in 30 minutes at 37°C.

 

Reaction Conditions

1X T7 DNA polymerase buffer, 1X BSA, Incubate at 37

 

Storage Conditions

50 mM KPO4, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.0 @ 25°C, Store at -20°C

 

Heat Inactivation

75°C for 20 min

 

Quality Control

- Endonuclease-free

 

Supplied with

- 10X T7 DNA Polymerase Buffer

- 100X BSA (10 mg/ml)

- Sterile water

 

Cautions

- Long incubation is unnecessary due to high polymerization rate of the enzyme.

- It is not suitable for DNA sequencing.


 

● Material Safety Data Sheet

- Enzynomics_MSDS_DP003_T7 DNA Polymerase

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