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2X TOPsimple™ PreMIX (aliquot)–nTaq

No Product Name Cat.# Conc Size Price(₩) Add
1 2X TOPsimple™ PreMIX (aliquot)–nTaq
(20 µl reactions)
P661T 2X 96 tubes ₩ 55,000 추가
2 2X TOPsimple™ PreMIX (aliquot)–nTaq
(20 µl reactions)
P662T 2X 960 tubes ₩ 440,000 추가
3 2X TOPsimple™ PreMIX (aliquot)–nTaq
(50 µl reactions)
P661T-50 2X 96 tubes ₩ 110,000 추가
4 2X TOPsimple™ PreMIX (aliquot)–nTaq
(50 µl reactions)
P662T-50 2X 960 tubes ₩ 880,000 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
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Product description

This product is 2X TOPsimple™ PreMIX–nTaq that is aliquoted into PCR tube strips. For example, 8 strips of 12 tubes are provided for 96 PCR reactions (Cat. P661T). 2X TOPsimple™ PreMIX–nTaq is a ready-to-use PCR mixtures containing 2X concentration of DNA polymerase, reaction buffer, and dNTP. This product includes nTaq (Cat. P025, P050), and is optimized for high efficiency PCR amplification with a high success rate. 

 

Characteristics

- Single-tube convenience

- Well-suited to analysis of a large number of samples.

- A-tail formation at 3’ ends of amplified duplex DNA

 

Applications

- Amplification of DNA fragments shorter than 3 kb (Suitable for general PCR analysis)

- Amplification of cDNA and genomic DNA.

- Primer extension

- Colony PCR

- Labeling of DNA fragments with radioactive-isotopes

- Nucleotide sequencing

 

Components (2X)

nTaq: 0.2 unit/µl

nTaq buffer (containing 3 mM MgCl2)

- dNTP mixture: 0.4 mM each

- Stabilizer

 

Storage

Stable up to 18 months at -20 or 3 months at 4 (Storage at -20 is recommended).

 

Standard reaction conditions

- PCR mixturea

Component

Reaction volume

(20 μl)

Reaction volume

(50 μl)

2X TOPsimple™ PreMIX(aliquot)-nTaq

1 tube

1 tube

Template DNAb (0.1~500 ng/μl)

1 μl

2.5 μl

Primer 1 (5 pmole/μl)

1 μl

2.5 μl

Primer 2 (5 pmole/μl)

1 μl

2.5 μl

Sterile water

up to 20 μl

up to 50 μl

aAssmeble the reaction mixture on ice

bPlasmid DNA, 0.1 ng-30 ng; genomic DNA, 50 ng-500 ng


- PCR cycles

Step

Temperature

Time

Cycle

Initial denaturation

95

2 min

1

Denaturation

95

30 sec

25~35

Annealinga

55~65

30~60 sec

Elongation

72

1 min/kb

Final elongation

72

5 min

1

When cycles are over, keep the reaction at 4; may add 10 mM EDTA until use to prevent DNA degradation

aRecommend annealing temperature is 5 to 10 below the lower Tm of the two primers used.


             



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