Recombinant mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) mature form produced in
Synonyms
GM-CSF, GMCSF, Colony-stimulating factor 2 (CSF-2), Molgramostim, Sargramostim
Expression Host
Escherichia coli (E. coli)
NCBI Accession Number
NP_034099.2
Amino Acid Sequence
Ala18-Lys141 with an N-terminal Methionine
N-terminal Sequence Analysis
Met-Ala-Pro-Thr-Arg (Minor : Ala-Pro-Thr-Arg-Ser)
Predicted Molecular Mass
14.2 kDa
Purity
> 98% by SDS-PAGE
Formulation
1.0 mg/ml mouse GM-CSF in PBS without carrier proteins
Sterility
Sterilized through a 0.22 µm membrane filter.
Biological Activity
The ED50 as determined by the dose-dependent stimulation of cell proliferation of FDC-P1 mouse bone marrow cell is 0.03~0.1 ng/ml.
Endotoxin Level
< 0.1 EU/μg of the protein as determined by the Limulus amebocyte lysate (LAL) method.
Shipping
The product is shipped with dry ice. Upon receipt, store it immediately at -80 °C before use.
Storage and Stability
This product can be diluted in sterile 1X PBS to obtain a concentration of 0.1 mg/ml and stored for a month at 4 °C. Prepare aliquots and freeze at -80 °C for long-term storage. Avoid repeated freeze-thaw cycles.
