DescriptionRecombinant human interleukin-13 (IL-13) mature form produced in E. coli
SynonymsIL-13, NC30, P600
Escherichia coli (E. coli)
NCBI Accession Number
Amino Acid SequenceLeu25-Asn146
N-terminal Sequence AnalysisLeu-Thr-Cys-Leu-Gly
Predicted Molecular Mass13.3 kDa
Purity > 97% by SDS-PAGE
Formulation 0.2 mg/ml human IL-13 in PBS without carrier proteins
Sterility Sterilized through a 0.22 µm membrane filter.
Biological ActivityThe ED50 as determined by the dose-dependent stimulation of cell proliferation of TF-1 human erythroleukemic cell is 2.7~6.8 ng/ml.
Endotoxin Level< 0.01 EU/μg of the protein as determined by the Limulus amebocyte lysate (LAL) method.
The product is shipped with dry ice. Upon receipt, store it immediately at -80 °C before use.
Storage and Stability
This product can be diluted in sterile 1X PBS to obtain a concentration of 0.1 mg/ml and stored for a month at 4 °C. Prepare aliquots and freeze at -80 °C for long-term storage. Avoid repeated freeze-thaw cycles.
Figure 1. SDS-PAGE
1 μg of Recombinant human IL-13 was
resolved with SDS-PAGE under reducing condition and visualized by Coomassie
Brilliant Blue staining.
Figure 2. Biological Activity
The biological activity of Recombinant
human IL-13 was measured by growth of TF-1 human erythroleukemic cells.