DescriptionRecombinant Human interleukin-6 (IL-6) mature form produced in E. coli
Synonyms IL-6, CTL differentiation factor, CDF, Hybridoma growth factor, HGF, HSF, B-cell stimulatory factor 2, BSF2, BSF-2, IFNB2, IFN-beta-2
Escherichia coli (E. coli)
NCBI Accession Number
Amino Acid Sequence
N-terminal Sequence Analysis
Predicted Molecular Mass
> 98% by SDS-PAGE
0.4 mg/ml human IL-6 in PBS without carrier proteins
Sterilized through a 0.22 µm membrane filter.
The ED50 as determined by the dose-dependent stimulation of cell proliferation of TF-1 human erythroleukemic cell is ≤ 0.2 ng/ml
< 0.1 EU/μg of the protein as determined by the Limulus amebocyte lysate (LAL) method.
The product is shipped with dry ice. Upon receipt, store it immediately at -80°C before use.
Storage and Stability
This product can be diluted in sterile 1X PBS to obtain a concentration of 0.1 mg/ml and stored for a month at 4 °C. Prepare aliquots and freeze at -80 °C for long-term storage. Avoid repeated freeze-thaw cycles.
of Recombinant human IL-6 was resolved with SDS-PAGE under reducing condition
and visualized by Coomassie Brilliant Blue staining.
Figure 2. Bioactivity assay
biological activity of Recombinant human IL-6 was measured by growth of TF-1
human erythroleukemic cells