interferon-γ (IFN-γ) mature form produced in E. coli
IFN-γ, IFNγ, IFN-gamma,
IFNgamma, Interferon-gamma, interferon gamma, IFNG, IFI, IFG
Escherichia coli (E.
NCBI Accession Number
Amino Acid Sequence
Gln24-Gln166, with an
> 98% by SDS-PAGE
1 mg/ml human IFN-γ in
PBS without carrier proteins
Sterilized through a
0.22 µm membrane filter.
The ED50 as
determined by the dose-dependent cytotoxic activity in the COLO205 human colon
adenocarcinoma cell is ≤ 4 ng/ml.
< 0.01 EU/μg of the
protein as determined by the Limulus amebocyte lysate (LAL) method.
The product is shipped
with dry ice. Upon receipt, store it immediately at -80°C before use.
Storage and Stability
product can be diluted in sterile 1X PBS to obtain a concentration of 0.1 mg/ml
and stored for a month at 4 °C. Prepare aliquots and freeze at -80 °C for
long-term storage. Avoid repeated freeze-thaw cycles.
Figure 1. SDS-PAGE
of Recombinant human IFN-γ
was resolved with SDS-PAGE under reducing condition and visualized by Coomassie
Figure 2. Bioactivity assay
activity of Recombinant human
human colon adenocarcinoma cells.