Human OSM

Product Name Cat. No. Size Conc.
Human OSM C005-10 10 μg 0.4 mg/ml
Human OSM C005-25 25 μg 0.4 mg/ml
Human OSM C005-100 100 μg 0.4 mg/ml
Human OSM C005-500 500 μg 0.4 mg/ml
Human OSM C005-1000 1 mg 0.4 mg/ml
리뷰 0 위시 0

상품간략정보 및 구매기능

Human OSM

상품 선택옵션 0 개, 추가옵션 0 개

제조사 C005-10
원산지 10 μg
브랜드 0.4 mg/ml
판매가격 232,000원
배송비결제 주문시 결제
  • Human OSM
    +0원

Description       

Recombinant Human Oncostatin M (OSM) mature form produced in E. coli

 

Synonyms         

OSM, MGC20461

 

Expression Host

Escherichia coli (E. coli)

 

Amino Acid Sequence    

Ala26-Arg221

 

N-terminal Sequence

Analysis Ala-Ala-Ile-Gly-Ser

 

Predicted Molecular Mass            

22.2 kDa

 

Purity   

> 95% by SDS-PAGE

 

Formulation      

0.4 mg/ml human OSM in PBS without carrier proteins.

 

Sterility

Sterilized through a 0.22 µm membrane filter.

 

Biological Activity          

The ED50 as determined by the dose-dependent stimulation of cell proliferation of TF-1 human erythroleukemic cell is ≤ 0.5 ng/ml.

 

Endotoxin Level

< 0.01 EU/μg of the protein as determined by the Limulus amebocyte lysate (LAL) method.

 

Shipping            

The product is shipped with dry ice. Upon receipt, store it immediately at -80 °C before use.

 

Storage and Stability      

This product can be diluted in sterile 1X PBS to obtain a concentration of 0.1 mg/ml and stored for a month at 4 °C. Prepare aliquots and freeze at -80 °C for long-term storage. Avoid repeated freeze-thaw cycles. 


 

Figure 1. SDS-PAGE

0.5 μg of Recombinant Human OSM was resolved with SDS-PAGE under reducing condition and visualized by Coomassie Brilliant Blue staining.

Figure 2. Bioactivity assay

The biological activity of recombinant human OSM was measured by growth of TF-1 human erythroleukemic cells. ED50 is ≤ 0.5 ng/ml. 

Certificates of analysis

Material Safety Data Sheet

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