Human Oncostatin M (OSM) mature form produced in E. coli
coli (E. coli)
95% by SDS-PAGE
mg/ml human OSM in PBS without carrier proteins.
through a 0.22 µm membrane filter.
ED50 as determined by the dose-dependent stimulation of cell proliferation of
TF-1 human erythroleukemic cell is ≤ 0.5 ng/ml.
0.01 EU/μg of the protein as determined by the Limulus amebocyte lysate (LAL)
product is shipped with dry ice. Upon receipt, store it immediately at -80 °C
product can be diluted in sterile 1X PBS to obtain a concentration of 0.1 mg/ml
and stored for a month at 4 °C. Prepare aliquots and freeze at -80 °C for
long-term storage. Avoid repeated freeze-thaw cycles.
of Recombinant Human OSM was resolved with SDS-PAGE under reducing condition
and visualized by Coomassie Brilliant Blue staining.
Figure 2. Bioactivity
The biological activity
of recombinant human OSM was measured by growth of TF-1 human erythroleukemic
cells. ED50 is ≤ 0.5 ng/ml.