Human Interleukin-18 (IL-18) mature form produced in E. coli
Interferon gamma-inducing factor, IGIF, IFN-gamma-inducing factor,
Interleukin-1 gamma, IL-1 gamma, Iboctadekin, IL1F4
coli (E. coli)
with an N-terminal Methionine
97% by SDS-PAGE
mg/ml human IL-18 in the storage buffer containing 50 mM Tris-HCl/pH7.8, 120 mM
NaCl, 0.69 mM ß-mercaptoethanol, 2.8% glycerol, 0.0027% NaN3, 0.027% Triton
X-100, and 0.027% NP-40 without carrier proteins.
through a 0.22 µm membrane filter.
ED50 as determined by the dose-dependent stimulation of IFN-γ secretion by KG-1
human acute myelogenous leukemia cells is ≤ 8 ng/ml.
0.1 EU/μg of the protein as determined by the Limulus amebocyte lysate (LAL)
product is shipped with dry ice. Upon receipt, store it immediately at -80 °C
product can be diluted in sterile 1X PBS to obtain a concentration of 0.1 mg/ml
and stored for a month at 4 °C. Prepare aliquots and freeze at -80 °C for
long-term storage. Avoid repeated freeze-thaw cycles.
2 μg of Recombinant
Human IL-18 was resolved with SDS-PAGE under reducing condition and visualized
by Coomassie Brilliant Blue staining.
Figure 2. Bioactivity
biological activity of recombinant human IL-18 was determined by the induction
of IFN-γ secretion by KG-1 cells. ED50
is ≤ 8 ng/ml.