Human IL-18

Product Name Cat. No. Size Conc.
Human IL-18 C004-10 10 µg 1 mg/ml
Human IL-18 C004-25 25 µg 1 mg/ml
Human IL-18 C004-100 100 µg 1 mg/ml
Human IL-18 C004-500 500 µg 1 mg/ml
Human IL-18 C004-1000 1 mg 1 mg/ml
리뷰 0 위시 0

상품간략정보 및 구매기능

Human IL-18

상품 선택옵션 0 개, 추가옵션 0 개

제조사 C004-10
원산지 10 µg
브랜드 1 mg/ml
판매가격 192,000원
배송비결제 주문시 결제
  • Human IL-18
    +0원

Description

Recombinant Human Interleukin-18 (IL-18) mature form produced in E. coli

 

Synonyms

IL-18, Interferon gamma-inducing factor, IGIF, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma, Iboctadekin, IL1F4

 

Expression Host

Escherichia coli (E. coli)

 

Amino Acid Sequence

Tyr37-Asp193, with an N-terminal Methionine

 

N-terminal Sequence

Analysis Met-Tyr-Phe-Gly-Lys

 

Predicted Molecular Mass            

18.3 kDa

 

Purity

> 97% by SDS-PAGE

 

Formulation

1 mg/ml human IL-18 in the storage buffer containing 50 mM Tris-HCl/pH7.8, 120 mM NaCl, 0.69 mM ß-mercaptoethanol, 2.8% glycerol, 0.0027% NaN3, 0.027% Triton X-100, and 0.027% NP-40 without carrier proteins.

 

Sterility

Sterilized through a 0.22 µm membrane filter.

 

Biological Activity

The ED50 as determined by the dose-dependent stimulation of IFN-γ secretion by KG-1 human acute myelogenous leukemia cells is ≤ 8 ng/ml.

 

Endotoxin Level

< 0.1 EU/μg of the protein as determined by the Limulus amebocyte lysate (LAL) method.

 

Shipping            

The product is shipped with dry ice. Upon receipt, store it immediately at -80 °C before use.

 

Storage and Stability      

This product can be diluted in sterile 1X PBS to obtain a concentration of 0.1 mg/ml and stored for a month at 4 °C. Prepare aliquots and freeze at -80 °C for long-term storage. Avoid repeated freeze-thaw cycles. 

Figure 1. SDS-PAGE

2 μg of Recombinant Human IL-18 was resolved with SDS-PAGE under reducing condition and visualized by Coomassie Brilliant Blue staining.

Figure 2. Bioactivity assay

The biological activity of recombinant human IL-18 was determined by the induction of IFN-γ secretion by KG-1 cells. ED50 is ≤ 8 ng/ml. 

Certificates of analysis

Material Safety Data Sheet

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