Human IL-3

Product Name Cat. No. Size Conc.
Human IL-3 C003-10 10 µg 1 mg/ml
Human IL-3 C003-25 25 µg 1 mg/ml
Human IL-3 C003-100 100 µg 1 mg/ml
Human IL-3 C003-500 500 µg 1 mg/ml
Human IL-3 C003-1000 1 mg 1 mg/ml
리뷰 0 위시 0

상품간략정보 및 구매기능

Human IL-3

상품 선택옵션 0 개, 추가옵션 0 개

제조사 C003-500
원산지 500 µg
브랜드 1 mg/ml
판매가격 2,340,000원
배송비결제 주문시 결제
  • Human IL-3
    +0원

Product description        

Recombinant Human Interleukin-3 (IL-3) mature form produced in E. coli

 

Synonyms         

IL-3, Multipotential colony-stimulating factor (MULTI-CSF), Mast cell growth factor (MCGF), Hematopoietic growth factor, P-cell-stimulating factor

 

Expression Host

Escherichia coli (E. coli)

 

NCBI Accession

Number  NP_000579.2

 

Amino Acid Sequence     

Ala20-Phe152, with an N-terminal Methionine

 

N-terminal Sequence Analysis     

Met-Ala-Pro-Met-Thr (Minor: Ala-Pro-Met-Thr-Gln)

 

Predicted Molecular Mass            

15.2 kDa

 

Purity   

> 98% by SDS-PAGE

 

Formulation       

1 mg/ml human IL-3 in PBS without carrier proteins

 

Sterility

Sterilized through a 0.22 µm membrane filter.

 

Biological Activity           

The ED50 as determined by the dose-dependent stimulation of cell proliferation of TF-1 human erythroleukemic cell is 0.05-1 ng/ml.

 

Endotoxin Level

< 0.1 EU/μg of the protein as determined by the Limulus amebocyte lysate (LAL) method.

 

Shipping            

The product is shipped with dry ice. Upon receipt, store it immediately at -80 °C before use.

 

Storage and Stability      

This product can be diluted in sterile 1X PBS to obtain a concentration of 0.1 mg/ml and stored for a month at 4 °C. Prepare aliquots and freeze at -80 °C for long-term storage. Avoid repeated freeze-thaw cycles. 

 

 

Figure 1. SDS-PAGE

1 μg of Recombinant human IL-3 was resolved with SDS-PAGE under reducing condition and visualized by Coomassie Brilliant Blue staining.



 

Figure 2. Bioactivity assay

The biological activity of Recombinant human IL-3 was measured by growth of TF-1 human erythroleukemic cells.
 

Certificates of analysis

Material Safety Data Sheet

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