Exonuclease-Resistant Random Primer is a mixture of single-stranded random oligonucleotides used for highly efficient random priming of various DNA synthesis reactions. It has two thiophosphate linkages at the 3' terminus to prevent digestion of the oligos by the 3' to 5' exonuclease activity of proofreading DNA polymerases, such as Phi29 Polymerase and Klenow Fragment. It also has 5'- and 3'-hydroxyl ends. The product is supplied as a ready-to-use, 20X concentrated aqueous solution.
3’-terminal PTO modifications protect from 3’→5’ exonuclease degradation and ensure efficient priming of DNA synthesis.
- Strand displacement amplification of DNA
- DNA labeling by random priming