TOPview™ ECL Pico Plus Western Substrate

Product Name Cat. No. Size Conc.
TOPview™ ECL Pico Plus Western Substrate EOE001S 50 ml *2 1 X
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상품간략정보 및 구매기능

TOPview™ ECL Pico Plus Western Substrate

상품 선택옵션 0 개, 추가옵션 0 개

제조사 EOE001S
원산지 50 ml *2
브랜드 1 X
판매가격 66,000원
배송비결제 주문시 결제
  • TOPview™ ECL Pico Plus Western Substrate
    +0원

Product description

TOPview™ ECL Pico Plus Western Substrate is a luminol-based chemiluminescent substrate featuring enhanced sensitivity. Compatible with immunoblots conducted with horseradish peroxidase (HRP) – conjugated secondary antibodies, it allows low-picogram or high-femtogram levels of antigen detection via its enhanced sensitivity and longer duration of chemiluminescent signal. This unique feature makes both digital- and film-based imaging possible without significant loss of the signal. Appropriate dilutions of primary and secondary antibody are recommended to obtain optimal intensity and duration of chemiluminescent signals

 

Characteristic

- High degree of sensitivity and long-lasting chemiluminescence signal.

- Optimized for use with PVDF and nitrocellulose membranes.

- Compatible with Western Blotting Markers.

- Optimized for film- and CCD-based imaging.

 

Storage Conditions

- Stable for up to 2 years at 4°C.

 

Standard Reaction Condition

1. Keep the membrane immersed in the wash buffer while preparing the substrate mixture solution. Please ensure that the membrane does not dry out during the subsequent steps.

2. Mix the luminol and peroxide solution in a 1:1 ratio, and thoroughly agitate the substrate mixture solution. The volume of mixture solution required is approximately 0.1 ml / cm2 of the membrane used.

- For a mini-sized membrane (7 x 8.5 cm), 5 ml of the mixture solution is sufficient.

- For a midi-sized membrane (8.5 x 13.5 cm), 10 ml of the mixture solution is sufficient.

3. Place the membrane with the protein side up on a level surface in a clean container.

4. Remove the membrane from the chemiluminescent substrate solution and drain off excess solution.

5. Place the membrane in a plastic sheet protector or in a plastic wrap to prevent the membrane from drying out.

6. Image the membrane with a digital imager or by exposing it to the X-ray film.

 

Troubleshooting

- High Background

Potential Causes

Solutions suggested

Overconcentrated primary

or secondary antibody

  Decrease the antibody concentration.

  Perform a dot blot to optimize the antibody concentration.

Insufficient wash

  Increase the number or intensity of Wash.

Incomplete blocking

  Decrease the antibody concentration.

  Perform a dot blot to optimize the antibody concentration.

 

- No Reaction or Weak Signal

Potential Causes

Solutions suggested

Insufficient antigen binding

  Increase antibody concentration.

  Optimize blocking reagents to achieve the balanced 

  sensitivity and specificity.

Poor antibody binding to the antigen

  Optimize the types and concentrations of detergent

  used. Increase the antibody incubation time.

Proteins washed from the

membrane during assay

  Reduce the number or intensity of wash.

Insufficient reagent volume

  Apply the additional volume of antibody blocking 

  reagent, or wash solution.

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