Cold UDG

Product Name Cat. No. Size Conc.
Cold UDG M062S 100 unit 1unit/μl
Cold UDG M062L 500 unit 1unit/μl
Cold UDG M062H 100 unit 5unit/μl
Cold UDG M062HL 500 unit 5unit/μl
리뷰 0 위시 0

상품간략정보 및 구매기능

Cold UDG

상품 선택옵션 0 개, 추가옵션 0 개

제조사 M062H
원산지 100 unit
브랜드 5unit/μl
판매가격 64,000원
배송비결제 주문시 결제
  • Cold UDG
    +0원

Product description

Cold UDG (Uracil-DNA Glycosylase) is quickly and irreversibly inactivated at mild heating condition (~50). The enzyme is engineered through super-computer assisted screening method. The hub amino acid residues important for maintaining structural stability of UDG were predicted by Laplacian clustering analysis. Iterative site-directed mutagenesis followed by expression and purification of recombinant engineered UDG were conducted. Finally, we select the most thermolabile UDG with the highest specific activity; Cold UDG. The enzyme can be applied for the prevention of carryover contamination in qPCR, and also in RT-qPCR.

 

Characteristics

- Heat inactivated at temperatures above 50°C

- Molecular weight: 24.5 kDa

- Reaction temperature: 37

 

Applications

- Control of carry-over contamination in PCR

- Cloning of PCR products

- Used to investigate features of protein-DNA interactions

 

Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free

- Phosphatase-free

 

Unit definition

One unit is defined as the amount of enzyme that catalyzes the release of 60 pmol of uracil per minute from double-stranded, uracil containing DNA

 

Storage buffer

10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.1 mg/ml BSA, 50% Glycerol pH 7.4 

Figure 1. Thermal inactivation of Cold UDG

Residual activity of Cold UDG and E.coli UDG after heat treatment at different temperature of 35~95°C. Cold UDG show great thermolability compare to E.coli UDG

 

Figure 2. Prevent PCR carryover contamination

qPCR assay was performed using serial diluted dU-containing PCR amplified DNA as a template. Cold UDG effectively removed dU-containing contaminated DNA and delayed cycle threshold from 7~9 (Red: w/o Cold UDG, Others: w/ cold UDG).
 

Certificates of analysis

Material Safety Data Sheet

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