Lambda Protein Phosphatase (Lamda PP)

Product Name Cat. No. Size Conc.
Lambda Protein Phosphatase (Lamda PP) M049S 20,000 unit 400 unit/μl
Lambda Protein Phosphatase (Lamda PP) M049M 40,000 unit 400 unit/μl
Lambda Protein Phosphatase (Lamda PP) M049L 100,000 unit 400 unit/μl
리뷰 0 위시 0

상품간략정보 및 구매기능

Lambda Protein Phosphatase (Lamda PP)

상품 선택옵션 0 개, 추가옵션 0 개

제조사 M049M
원산지 40,000 unit
브랜드 400 unit/μl
판매가격 279,000원
배송비결제 주문시 결제
  • Lambda Protein Phosphatase (Lamda PP)
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Product description

Full-length, Lambda Protein Phosphatase (Lambda PP) is the 221 amino-acid product from ORF221 of bacteriophage lambda. Lambda PP is a Mn2+-dependent protein phosphatase with activity towards phosphorylated serine, threonine, tyrosine and histidine residues. Lambda PP is expressed and purified from E. coli as a recombinant.

 

Characteristics

- Molecular weight: 25 kDa

- Reaction temperature: 30

- Heat inactivation: 65 for 1 hr in the presence of 50 mM EDTA

 

Applications

Lambda Protein Phosphatase can be used to release phosphate groups from phosphorylated serine, threonine and tyrosine residues in proteins.

 

Quality control

- Purity: >95% on SDS-PAGE

- Protease-free

 

Supplied with

- 10X Lambda PP Buffer

- 10X MnCl2

 

Unit definition

One unit will hydrolyze 1 nmole of p-nitrophenyl phosphate per minute at 30 and pH 7.5.

 

Storage buffer

50 mM HEPES, 100 mM NaCl, 2 mM DTT, 0.1 mM EDTA, 0.1 mM MnCl2, 0.01% Brij 35, 50% glycerol, pH 7.5

 

Cautions

- Repeated freeze/thaw cycles is not recommended.  

- For each individual substrate, optimal incubations times and enzyme concentrations must be established experimentally in advance.

- In case of assays on crude samples, addition of protein inhibitor cocktail and shorter incubation time are recommended.

- The phosphatase activity is inhibited at the high concentrations of sodium orthovanadate (> 50mM), sodium fluoride (> 50 mM) and EDTA (> 50 mM).  

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