Full-length, Lambda Protein Phosphatase (Lambda PP) is the 221 amino-acid product from ORF221 of bacteriophage lambda. Lambda PP is a Mn2+-dependent protein phosphatase with activity towards phosphorylated serine, threonine, tyrosine and histidine residues. Lambda PP is expressed and purified from E. coli as a recombinant.
- Molecular weight: 25 kDa
- Reaction temperature: 30℃
- Heat inactivation: 65℃ for 1 hr in the presence of 50 mM EDTA
Lambda Protein Phosphatase can be used to release phosphate groups from phosphorylated serine, threonine and tyrosine residues in proteins.
- Purity: >95% on SDS-PAGE
- 10X Lambda PP Buffer
- 10X MnCl2
One unit will hydrolyze 1 nmole of p-nitrophenyl phosphate per minute at 30℃ and pH 7.5.
50 mM HEPES, 100 mM NaCl, 2 mM DTT, 0.1 mM EDTA, 0.1 mM MnCl2, 0.01% Brij 35, 50% glycerol, pH 7.5
- Repeated freeze/thaw cycles is not recommended.
- For each individual substrate, optimal incubations times and enzyme concentrations must be established experimentally in advance.
- In case of assays on crude samples, addition of protein inhibitor cocktail and shorter incubation time are recommended.
- The phosphatase activity is inhibited at the high concentrations of sodium orthovanadate (> 50mM), sodium fluoride (> 50 mM) and EDTA (> 50 mM).