EZ™ Adaptor Ligation Module is formulated for quickly ligate DNA adaptors to blunt or dA-Tailed DNA fragments. The product provides efficient performance and room temperature reaction setup in a convenient master mix.
Master mix type ligase for DNA adaptor ligation
- Ligate DNA adaptors to blunt or dA-tailed DNA fragments
- DNA Sample Preparation for NGS
- Ligation of blunt or dA-Tailed DNA and cloning vectors.
Figure 1. The result of adaptor ligation reaction
30-nt dT-tailed dsDNA adaptors were covalently attached to 20-nt dA-tailed dsDNA using EZTM Adaptor Ligation Module. Reaction mixtures were incubated at 25℃ for 15 min, followed by analyzed on 10% denaturing PAGE.