JM109 Chemically Competent E. coli

Product Name Cat. No. Size Conc.
JM109 Chemically Competent E. coli CP910 1.1 ml -
JM109 Chemically Competent E. coli CP911 2.1 ml -
리뷰 0 위시 0

상품간략정보 및 구매기능

JM109 Chemically Competent E. coli

상품 선택옵션 0 개, 추가옵션 0 개

제조사 CP910
원산지 1.1 ml
브랜드 -
판매가격 60,000원
배송비결제 주문시 결제
  • JM109 Chemically Competent E. coli
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Product description

This product contains highly efficient competent cells prepared from E. coli JM109. They can be transformed by supercoiled plasmid DNA (pUC19) with a high efficiency (1 x 108 cfu/μg). This product is appropriate for plasmid amplification, transformation of ligated DNA and general cloning.

 

Characteristics

- Strain: JM109

- Genotype: F’ traD36 proA+ proB+ lacIq (lacZ)M15 (lac-proAB) supE44 hsdR17 recA1 gyrA96 thi-1 endA1 relA1 e14- λ-

 

Applications

- Amplification and purification of plasmid DNAs

- Construction of genomic DNA and cDNA libraries

- General cloning

 

Quality control

- Transformation efficiency: ~1 x 108 cfu/μg of pUC19

 

Cautions

- It is appropriate to use such antibiotics at their recommendable concentrations: ampicillin (50 μg/ml), kanamycin (25 μg/ml), and tetracycline (12.5 μg/ml)

- Higher antibiotic concentrations will cause decreased transfection efficiency while lower antibiotic concentrations will cause the increased number of satellite colonies. 

- False-positive colonies can appear if cells are cultured at 37 for 18-24 hr.  

Certificates of analysis

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