Rosett(DE3) Chemically Competent E.coli

Product Name Cat. No. Size Conc.
Rosett(DE3) Chemically Competent E.coli CP1010 1.1 ml -
Rosett(DE3) Chemically Competent E.coli CP1011 2.1 ml -
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상품간략정보 및 구매기능

Rosett(DE3) Chemically Competent E.coli

상품 선택옵션 0 개, 추가옵션 0 개

제조사 CP1011
원산지 2.1 ml
브랜드 -
판매가격 240,000원
배송비결제 주문시 결제
  • Rosett(DE3) Chemically Competent E.coli
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Product description

 This product contains highly efficient competent cells prepared from E. coli Rosett(DE3). The competent cells can be transformed by supercoiled plasmid DNA (pUC19) with high efficiency. This product can be used for general cloning, plasmid amplification, and transformation using ligated DNA. The convenient and efficient construction of plasmid recombinants can be done with these competent cells, which are grown and then made chemically competent via an optimized procedure before the verification of cloning efficiency and strain identity. This product also can be used for making new expression host with other genetic backgrounds.  As BL21 derivatives genetically designed to improve the expression of eukaryotic genes that contain codons rarely used in E. coli, Rosett host strain strains supply tRNAs for AGG, AGA, AUA, CUA, CCC, GGA codons via a compatible chloramphenicol -resistant plasmid. As a result, the Rosett strains facilitate “universal” translation which is otherwise limited by the codon usage of E. coliDE3 signifies that the host is a lysogen of λDE3, which has a chromosomal copy of the T7 RNA polymerase gene controlled by the lacUV5 promoter. Such strains are appropriate for the protein expression from target genes cloned in pET vectors via IPTG induction.

 

Characteristics

- Strain: Rosetta(DE3) Chemically Competent E. coli

- Genotype: F- ompT hsdSB(rB- mB-) gal dcm (DE3) pRARE (CamR)

 

Applications

- Amplification and purification of plasmid DNAs

- Construction of genomic DNA and cDNA libraries

- General cloning

 

Quality control

- Transformation efficiency: ≥ 3.0 x 106 cfu/μg of pUC19 

Certificates of analysis

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