EZchange™ Multi Site-directed Mutagenesis core kit

Product Name Cat. No. Size Conc.
EZchange™ Multi Site-directed Mutagenesis core kit EZ024S 10 rxn -
EZchange™ Multi Site-directed Mutagenesis core kit EZ024M 20 rxn -
리뷰 0 위시 0

상품간략정보 및 구매기능

EZchange™ Multi Site-directed Mutagenesis core kit

상품 선택옵션 0 개, 추가옵션 0 개

제조사 EZ024S
원산지 10 rxn
브랜드 -
판매가격 250,000원
배송비결제 주문시 결제
  • EZchange™ Multi Site-directed Mutagenesis core kit
    +0원

Product description
EZchange™ Multi site-directed mutagenesis Kit is designed to create multiple site-directed mutations in plasmid DNA. The product consists of three processes: amplification, DpnI digestion, and transformation. Step 1, Mutagenic primers are annealed to denatured template plasmid. Each primer has the desired sequence changes. DNA polymerase extends the oligonucleotides and ligase seals the DNA strands creating a replicated plasmid possessing the desired mutation. This process is repeated in PCR amplification for 30 cycles. In step 2, PCR amplification reaction is treated Dpn I endonuclease, it cleaves methylated and hemi-methylated DNA. In step3, Dpn I reaction mixture is transformed into competent cells, where ssDNA is converted into dsDNA. This simple and quick procedure provides high mutagenesis efficiency greater than 50%, even with multiple mutations.

 

Kits contents

- EZchange™ Multi Enzyme mixture

- EZchange™ Multi 10X Reaction buffer

- dNTP mixture (2 mM each)

- EZchange™ Multi Control plasmid (50 ng/μl)

- EZchange™ Multi Control primer mixture (10 pmol/μl each of three primers)

- Dpn I restriction endonuclease (20 units/μl)

- Sterile water

 

Reagents not provided in the Kit

- DH5α Chemically Competent E. coli

- X-gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside)

- IPTG (Isopropyl β-D-1-thiogalactopyranoside)

 

Storage conditions

- 10X reaction buffer, DH5α competent cell: -80 

- All other component: -20 

 

Note

- Avoid defrost cycle of reaction buffer and dNTP mixture. We recommend make small aliquots, each for a single use.

- Template plasmids should be purified from dam+ E. coli strains. Plasmid DNA isolated from the exceptional   dam- E.coli strains, including JM110 and SCS110 is not suitable, since they are not digested by Dpn I.  

- This kit is not recommended for use with template plasmid greater than 5 kb.  

Certificates of analysis

  • 등록된 내용이 없습니다.

Products related to the selected product.

선택하신 상품이 장바구니에 담겼습니다.

계속구매 장바구니 이동
  • Add.

    281-9, Munji-ro, Yuseong-gu, Daejeon, 34050, Republic of Korea

  • Tel. +82 - 42 - 719 - 1023
  • Fax. +82 - 42 - 719 - 1024
  • Email. info@enzynomics.com

copyright© Enzynomics co Ltd, All rights reserved.

상단으로