TOPcloner™ XL TA Kit

Product Name Cat. No. Size Conc.
TOPcloner™ XL TA Kit EZ033S 10 rxn -
TOPcloner™ XL TA Kit EZ033M 20 rxn -
리뷰 0 위시 0

상품간략정보 및 구매기능

TOPcloner™ XL TA Kit
제조사 EZ033S
원산지 10 rxn
브랜드 -
판매가격 판매중지
배송비결제 주문시 결제

Product description

TOPcloner™ XL TA Kit is suitable for cloning Taq polymerase-amplified long (3-10 kb) PCR products (with A-tail overhangs) at room temperature in 5 min. The vector provided in this Kit contains topoisomerase covalently coupled to its ends, which allows efficient ligation of DNA fragments with A-tail overhangs to the vector without using a regular ligase. This kit provides special gel purification reagents that ensures efficient cloning of long, full-length PCR products.

 

Characteristics

- Highly efficient long PCR product (3-10 kb) cloning

- Short reaction time (5 min)

- Direct use of PCR products

 

Applications

- Fast and efficient cloning of long, full-length (3-10 kb) A-tailed PCR products.

  

Component

Box 1

  pTOP TA V2, 6X TOPcloner buffer, M13 reverse primer, M13

  forward primer Control insert, Control primers mix, Sterile water

Box 2

  S.O.C. Media, DH5α chemical competent E.coli, pUC19 control DNA

Box 3

  Buffer GB, Buffer NW, Buffer EB, Crystal Violet, 6XCrystal Violet Loading Buffer

 

Figure 1. A schematic map of pTOP TA V2 (3807 bp). 

A molecule of topoisomerase (TOPO) is covalently coupled to each of the two 3 one-nt protruding ends (nt positions 294 and 295) of pTOP TA V2 (Cat.# EZ001 and EZ011). Therefore, pTOP TA V2 is ready to form a covalent bond when mixed with PCR products with one-nt 3 A overhang. The addition of one A to the duplex DNA ends is intrinsic to Taq DNA polymerase.

 

Figure 2. The result of colony PCR. 

Large (10 kb) size of PCR product was cloned using TOPcloner™ XL TA Kit. 

 

Certificates of analysis

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