TOPcloner™ TA-Blunt kit

Product Name Cat. No. Size Conc.
TOPcloner™ TA-Blunt kit EZ007S 20 rxn -
TOPcloner™ TA-Blunt kit EZ007M 40 rxn -
리뷰 0 위시 0

상품간략정보 및 구매기능

TOPcloner™ TA-Blunt kit

상품 선택옵션 0 개, 추가옵션 0 개

제조사 EZ007S
원산지 20 rxn
브랜드 -
판매가격 250,000원
배송비결제 주문시 결제
  • TOPcloner™ TA-Blunt kit
    +0원

Product description

TOPcloner™ TA-Blunt kit are suitable for cloning at room temperature in 5 min with both Taq polymerase-amplified PCR products (A tail overhangs) and Pfu polymerase-amplified PCR products (blunt ends). The vector provided in this kit contains topoisomerase covalently coupled to its ends, which allows efficient cloning of any DNA fragments to the vector without using a regular ligase.

 

Characteristics

- Short reaction time (5 min)

- Highly efficient cloning

- Direct use of PCR products

 

Applications

- Fast and efficient cloning of both A-tailed and blunt ended PCR products

 

Supplied with

- pTOP TA-Blunt V2

- 6X TOPcloner™ Buffer

- 2X TOPsimple ™ DyeMIX-Tenuto

- M13F, M13R (10 pmol/μl)

- Control insert (TA) (20 ng/μl)

- Control insert (Blunt) (20 ng/μl)

- Control primers (10 pmol/μl each)

- DH5α Chemically Competent E. coli

- Sterile water

 

Quality control

- Cloning efficiency: 99%

Certificates of analysis

Products related to the selected product.

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