TOPcloner™ Blunt kit

Product Name Cat. No. Size Conc.
TOPcloner™ Blunt kit EZ002S 20 rxn -
TOPcloner™ Blunt kit EZ002M 40 rxn -
리뷰 0 위시 0

상품간략정보 및 구매기능

TOPcloner™ Blunt kit

상품 선택옵션 0 개, 추가옵션 0 개

제조사 EZ002S
원산지 20 rxn
브랜드 -
판매가격 250,000원
배송비결제 주문시 결제
  • TOPcloner™ Blunt kit
    +0원

Product description

TOPcloner™ Blunt kit is suitable for cloning Pfu polymerase-amplified PCR products with blunt ends at room temperature in 5 min. The vector provided in this kit contains topoisomerase covalently coupled to its ends, which allows efficient cloning of blunt-ended DNA fragments to the vector without using a regular ligase.

 

Characteristics

 - Short reaction time (5 min)

 - Highly efficient cloning

 - Direct use of PCR products

 

Applications

 - Fast and efficient cloning of blunt-ended PCR products (for example, Pfu-amplified DNA)

 

Supplied with

- pTOP Blunt V2

- 6X TOPcloner™ Buffer

- 2X TOPsimple ™ DyeMIX-Tenuto

- DH5α Chemically Competent E. coli

- M13F, M13R (10 pmol/μl)

- Control insert DNA (20 ng/μl)

- Control primers (10 pmol/μl each)

- Sterile water

 

Quality control

 - Cloning efficiency: 99% 

  

 

Figure. A schematic map of pTOP Blunt V2 (3807 bp).

A molecule of topoisomerase (TOPO) is covalently coupled to the 3' blunt duplex ends (nt positions 294 and 295) of pTOP Blunt V2 (Cat.# EZ002 and EZ012). Therefore, pTOP Blunt V2 can form a covalent bond with blunt-ended PCR products obtained by Pfu DNA polymerase.

 - Lac promoter/operator : 95-216
 - M13 Reverse Primer binding site : 205-221
 - LacZ ORF : 217-534
 - MCS, Multiple Cloning Sites : 234-357
 - M13 (-20) Forward Primer binding site : 391-406
 - ccdB ORF : 544-846
 - Kanr gene : 1057-1989
 - Ampr gene : 2007-2867 

 - pUC origin : 3012-3685 

Certificates of analysis

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