TOPcloner™ TA kit

Product Name Cat. No. Size Conc.
TOPcloner™ TA kit EZ001S 20 rxn -
TOPcloner™ TA kit EZ001M 40 rxn -
리뷰 0 위시 0

상품간략정보 및 구매기능

TOPcloner™ TA kit

상품 선택옵션 0 개, 추가옵션 0 개

제조사 EZ001S
원산지 20 rxn
브랜드 -
판매가격 250,000원
배송비결제 주문시 결제
  • TOPcloner™ TA kit
    +0원

Product description

TOPcloner™ TA kit is suitable for cloning Taq polymerase-amplified PCR products with A-tail overhangs at room temperature in 5 min. The vector provided in this kit contains topoisomerase covalently coupled to its ends, which allows efficient cloning of DNA fragments with A-tail overhangs to the vector without using a regular ligase.

 

Characteristics

- Short reaction time (5 min)

- Highly efficient cloning

- Direct use of PCR products

 

Applications

- Fast and efficient cloning of A-tailed PCR products (for example, Taq-amplified DNA)

 

Supplied with

- pTOP TA V2

- 6X TOPcloner™ Buffer

- 2X TOPsimple ™ DyeMIX-Tenuto

- M13F, M13R (10 pmol/μl)

- Control insert DNA (20 ng/μl)

- Control primers (10 pmol/μl each)

- DH5α Chemically Competent E. coli

- Sterile water

 

Quality control

- Cloning efficiency: 99% 

 


Figure. A schematic map of pTOP TA V2 (3807 bp).
 A molecule of topoisomerase (TOPO) is covalently coupled to each of the two 3' one-nt protruding ends (nt positions 294 and 295) of pTOP TA V2 (Cat.# EZ001 and EZ011). Therefore, pTOP TA V2 is ready to form a covalent bond when mixed with PCR products with one-nt 3' A overhang. The addition of one A to the duplex DNA ends is intrinsic to Taq DNA polymerase.

 - Lac promoter/operator : 95-216
 - M13 Reverse Primer binding site : 205-221
 - LacZ ORF : 217-534
 - MCS, Multiple Cloning Sites : 234-357
 - M13 (-20) Forward Primer binding site : 391-406
 - ccdB ORF : 544-846
 - Kanr gene : 1057-1989
 - Ampr gene : 2007-2867 

 - pUC origin : 3012-3685

 

Certificates of analysis

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