TOPreal™ qPCR 2X PreMIX (TaqMan Probe for multiplex)

Product Name Cat. No. Size Conc.
TOPreal™ qPCR 2X PreMIX (TaqMan Probe for multiplex) RT620S 200 rxn 2 X
TOPreal™ qPCR 2X PreMIX (TaqMan Probe for multiplex) RT620M 500 rxn 2 X
리뷰 0 위시 0

상품간략정보 및 구매기능

TOPreal™ qPCR 2X PreMIX (TaqMan Probe for multiplex)

상품 선택옵션 0 개, 추가옵션 0 개

제조사 RT620S
원산지 200 rxn
브랜드 2 X
판매가격 150,000원
배송비결제 주문시 결제
  • TOPreal™ qPCR 2X PreMIX (TaqMan Probe for multiplex)
    +0원
● Rox final concentration for different qPCR instruments


Product description

TOPreal™ qPCR 2X PreMIX (TaqMan Probe for multiplex) is a ready-to-use PCR mixture containing 2X concentration of DNA polymerase, reaction buffer, and dNTP. The resulting hot-start PCR amplification provides increased sensitivity and specificity. An optimized multiplex PCR master mix designed specifically for Real-time PCR, which makes it possible to detect multiple targets at the same time. This kit provides a level of sensitivity equally or superior than singleplex probe-based real-time PCR. Additionally, this kit minimized the fluorescence signal decrement derived from probe to probe and primer to primer interference.

 

Characteristics

- Multiplexing

- Ease of Use: Premix contains everything needed except for primers, template and probe.

- High specificity: Enhanced specificity by the use of HOT Start PCR polymerase.

- Stability: Enhanced stability allows one year storage at -20

- Included 100 nM ROX reference Dye

 

Applications

- Pathogen Identification

- High Throughput SNP Genotyping

- Gene Deletion Analysis

- Template Quantitation

- Linkage Analysis 

 

Figure 1. Multiplex detection of various target.

Three different targets were simultaneously amplified with TOPreal™ qPCR 2X PreMIX (TaqMan Probe for multiplex) using 10-fold serially diluted purified DNAs as templates. The primer/probe sets (FAM, Cy5, VIC) were specifically designed for each target and real-time PCR was conducted with mixed oligonucleotides in a single reaction tube for multiplex detection.

Red: Cy5 detection / Green: FAM detection / Black: VIC detection
 

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