TOPscript™ III Reverse Transcriptase (High fidelity)

Product Name Cat. No. Size Conc.
TOPscript™ III Reverse Transcriptase (High fidelity) RT004S 10,000 unit 200 unit/μl
TOPscript™ III Reverse Transcriptase (High fidelity) RT004M 20,000 unit 200 unit/μl
TOPscript™ III Reverse Transcriptase (High fidelity) RT004L 50,000 unit 200 unit/μl
TOPscript™ III Reverse Transcriptase (High fidelity) RT004H 50,000 unit 1,000 unit/μl
리뷰 0 위시 0

상품간략정보 및 구매기능

TOPscript™ III Reverse Transcriptase (High fidelity)

상품 선택옵션 0 개, 추가옵션 0 개

제조사 RT004S
원산지 10,000 unit
브랜드 200 unit/μl
판매가격 144,000원
배송비결제 주문시 결제
  • TOPscript™ III Reverse Transcriptase (High fidelity)
    +0원

Product description

TOPscript™ III Reverse Transcriptase (High fidelity) is a blend of a recombinant reverse transcriptase and a proofreading enzyme. The synergistic effect of both enzymes generate 7-fold higher fidelity compared to other commonly used reverse transcriptases. Due to the higher thermostability of reverse transcriptase and the specially optimized buffer system, reverse transcription reaction is possible at temperatures up to +55TOPscript™ III Reverse Transcriptase (High fidelity) does not degrade RNA strands from RNA-DNA hybrids and therefore is capable of high yield of synthesizing cDNA. The optimum temperature of TOPscript™ III Reverse Transcriptase (High fidelity) is 50TOPscript™ III Reverse Transcriptase (High fidelity) does not show inhibitory effect on one-step RT-PCR reaction.

 

Caracteristics

- Thermostable and RNase H variant of M-MLV RTase

- Synthesize cDNA at 42~55.

- Especially the highest activity at 50

- Generate full-length transcripts up to 14 kb with the oligo(dT) primers.

- Increase accuracy during reverse transcription reactions.

- Efficiently synthesize cDNA by inhibiting the formation of secondary structure of RNA.

 

Applications

- Cloning genes of interest

- Sequencing transcriptomes

- Generating cDNA libraries with large and full-length inserts

- Generating gene expression analysis, like RNA splicing analysis.

 

Unit definition

One unit is the amount of enzyme required to incorporate 1 nmol dTTP into acidinsoluble materials using 0.4 mM poly(rA)-oligo(dT) as substrate at 37 in 10 min.

 

Supplied with:

- 10X TOPscript™ RT Buffer

- dNTP Mixture (2 mM each)

- Sterile water (RNase free)

 

Storage buffer

20 mM Tris-Cl (pH 7.5), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.01% NP40, 50% glycerol

 

10X TOPscript™ RT Buffer

500 mM Tris-HCl (pH 8.3), 30 mM MgCl, 100 mM DTT, 750 mM KCl 

  

Note 

TOPscript™ Reverse Transcriptase performs optionally over the full range of 42-60. Typically, 50 is a good starting point. For RNAs containing secondary structure and other challenging targets, a synthesis temperature of 60 may be used without loss of performance. 

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