Bst DNA Polymerase (Large Fragment)

Product Name Cat. No. Size Conc.
Bst DNA Polymerase (Large Fragment) DP006S 1,600 unit 8 unit/μl
Bst DNA Polymerase (Large Fragment) DP006L 8,000 unit 8 unit/μl
Bst DNA Polymerase (Large Fragment) DP006H 8,000 unit 100 unit/μl
리뷰 0 위시 0

상품간략정보 및 구매기능

Bst DNA Polymerase (Large Fragment)

상품 선택옵션 0 개, 추가옵션 0 개

제조사 DP006S
원산지 1,600 unit
브랜드 8 unit/μl
판매가격 68,000원
배송비결제 주문시 결제
  • Bst DNA Polymerase (Large Fragment)
    +0원

Product description

Large Fragment of the Bst DNA Polymerase from Bacillus stearothermophilus is isolated as a recombinant. It lacks the 5´ → 3´ exonuclease domain. Therefore, Large Fragment of the Bst DNA Polymerase catalyzes 5' → 3' synthesis of DNA and but lacks 5' → 3' and 3' → 5' exonuclease activities.

 

Characteristic

- Isolated from a recombinant source

- Sequencing through problematic secondary structures

- Thermophilic DNA polymerase with strong strand displacement activity

 

Activity

- 5’→3’ exonuclease: No

- 3’→5’ exonuclease: No

- Strand Displacement: Yes

 

Applications

- Random-primed DNA labeling

- Labeling by fill-in 5'-overhangs of dsDNA

- Loop-mediated isothermal amplification (LAMP).

- Whole genome amplification (WGA).

- Ramification amplification (RAM).

 

Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free

- RNase-free

 

Unit definition 

One unit is defined at the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.

Storage condition

10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.1% Triton® X-100, pH 7.4 @ 25°C

 

1X Reaction buffer

20 mM Tris-HCl, 10 mM (NH4)2SO4, 10 mM KCl, 2 mM MgSO4, 0.1% Triton® X-100, pH 8.8 @ 25°C

 

Supplied with

- 10X Bst DNA Polymerase(Large) Buffer

- 100mM MgSO4

- Sterile water 

Certificates of analysis

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