EZ-CleanCut™ Nhe I

Product Name Cat. No. Size Conc.
EZ-CleanCut™ Nhe I CR016S 1,000 unit 10 unit/μl
EZ-CleanCut™ Nhe I CR016M 2,000 unit 10 unit/μl
EZ-CleanCut™ Nhe I CR016L 5,000 unit 10 unit/μl
EZ-CleanCut™ Nhe I CR016H 5,000 unit 50 unit/μl
리뷰 0 위시 0

상품간략정보 및 구매기능

EZ-CleanCut™ Nhe I

상품 선택옵션 0 개, 추가옵션 0 개

제조사 CR016S
원산지 1,000 unit
브랜드 10 unit/μl
판매가격 68,000원
배송비결제 주문시 결제
  • EZ-CleanCut™ Nhe I
    +0원

 EZ-CleanCut™ Nhe I with high purity and fidelity has greatly reduced star activity than Nhe I. 

 

Recognition & cleavage sequence

 


 

SourceNeisseria mucosa heidelbergensis

 

Unit definition

One unit is defined as the amount of enzyme required for complete digestion of 1-μg bacteriophage λ DNA at 37 for 1 hr in a 50-μl reaction mixture.

 

Reaction conditions

- 1X EzBuffer IV, 37

 

Thermal inactivation

Temperature

Time

80

20 min

 

Methylation effect

Methylation

dam

dcm

CpG

Cleavage

Cleavage

Cleavage

Conditional

 

Enzyme activity in EzBuffer

I

II

III

IV

FastCut

100%

25%

10%

100%

NR

 

Product component

- EZ-CleanCut™ Nhe I

- 10X EzBuffer IV

- 6X DNA loading Buffer

- Sterile water

 

Note

EZ-CleanCut™ Nhe I cleaves DNA and then leaves a 5' CTAG extension, which can be efficiently ligated to DNA fragments cleaved by Avr II, Spe I, or Xba I. Cleavage of mammalian genomic DNA is prevented by CpG methylation partially overlapping the recognition sequence. Salt concentrations above 100 mM inhibit enzyme activity.

 

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