EcoO109 I

Product Name Cat. No. Size Conc.
EcoO109 I R085S 2,000 unit 20 unit/μl
EcoO109 I R085M 4,000 unit 20 unit/μl
EcoO109 I R085L 10,000 unit 20 unit/μl
EcoO109 I R085H 10,000 unit 100 unit/μl
리뷰 0 위시 0

상품간략정보 및 구매기능

EcoO109 I

상품 선택옵션 0 개, 추가옵션 0 개

제조사 R085H
원산지 10,000 unit
브랜드 100 unit/μl
판매가격 268,000원
배송비결제 주문시 결제
  • EcoO109 I
    +0원

Recognition & cleavage sequence

 


 

SourceE. coli H709c

 

Unit definition

One unit is defined as the amount of enzyme required for complete digestion of 1 μg bacteriophage λ DNA at 37for 1 hr in a 50 μl reaction mixture.

 

Reaction condition

- 1X EzBuffer IV, 37

- 1X FastCut Buffer, 37

 

Thermal inactivation

Temperature

Time

65

20 min

 

Methylation effect

Methylation

dam

dcm

CpG

Cleavage

Cleavage

Conditional

Cleavage

 

Enzyme activity in EzBuffer

I

II

III

IV

FastCut

50%

75%

100%

100%

100%

 

Product component

- EcoO109 I

- 10X EzBuffer IV

- 10X FastCut Buffer

- 6X DNA loading Buffer

- Sterile water

 

Note

EcoO109 I is an isoschizomer of Dra . Activity is inhibited by dcm methylation partially overlapping its recognition. 


 

Figure 1. Unit definition

One unit is defined as the amount of enzyme required to digest 1 µg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 µl. 

Certificates of analysis

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