Sac I

Product Name Cat. No. Size Conc.
Sac I R005S 2,000 unit 20 unit/μl
Sac I R005M 4,000 unit 20 unit/μl
Sac I R005L 10,000 unit 20 unit/μl
Sac I R005H 10,000 unit 100 unit/μl
리뷰 0 위시 0

상품간략정보 및 구매기능

Sac I

상품 선택옵션 0 개, 추가옵션 0 개

제조사 R005M
원산지 4,000 unit
브랜드 20 unit/μl
판매가격 122,400원
배송비결제 주문시 결제
  • Sac I
    +0원

Recognition & cleavage sequence




SourceStreptomyces achromogenes

 

Unit definition

One unit is defined as the amount of enzyme required for complete digestion of 1-μg bacteriophage λ DNA at 37for 1 hr in a 50-μl reaction mixture.

 

Reaction conditions

- 1X EzBuffer I, 37

- 1X FastCut Buffer, 37

 

Thermal inactivation

Temperature

Time

65

20 min

 

Methylation effect

Methylation

dam

dcm

CpG

Cleavage

Cleavage

Cleavage

Cleavage

 

Enzyme activity in EzBuffer

I

II

III

IV

FastCut

100%

75%

25%

75%

100%

 

Product component

- Sac I

- 10X EzBuffer I

- 10X FastCut Buffer

- 6X DNA loading Buffer

- Sterile water

 

Note

High salt concentrations above 10 mM inhibit enzyme activity. Use clean DNA for efficient cleavage. It is not affected by damdcm, or mammalian CpG methylation. 


 

Figure 1. Unit definition

One unit is defined as the amount of enzyme required to digest 1 µg of Lambda Hind III in 1 hour at 37°C in a total reaction volume of 50 µl. 

Certificates of analysis

  • 등록된 내용이 없습니다.

Products related to the selected product.

선택하신 상품이 장바구니에 담겼습니다.

계속구매 장바구니 이동
  • Add.

    281-9, Munji-ro, Yuseong-gu, Daejeon, 34050, Republic of Korea

  • Tel. +82 - 42 - 719 - 1023
  • Fax. +82 - 42 - 719 - 1024
  • Email. info@enzynomics.com

copyright© Enzynomics co Ltd, All rights reserved.

상단으로