Nco I

Product Name Cat. No. Size Conc.
Nco I R004S 1,000 unit 10 unit/μl
Nco I R004M 2,000 unit 10 unit/μl
Nco I R004L 5,000 unit 10 unit/μl
Nco I R004H 5,000 unit 50 unit/μl
리뷰 0 위시 0

상품간략정보 및 구매기능

Nco I

상품 선택옵션 0 개, 추가옵션 0 개

제조사 R004M
원산지 2,000 unit
브랜드 10 unit/μl
판매가격 122,400원
배송비결제 주문시 결제
  • Nco I
    +0원

Recognition & cleavage sequence




SourceNocardia corallina

 

Unit definition

One unit is defined as the amount of enzyme required for complete digestion of 1 μg bacteriophage λ DNA at 37 for 1 hr in a 50 μl reaction mixture.

 

Reaction condition

- 1X EzBuffer III, 37

- 1X FastCut Buffer, 37

 

Thermal inactivation

Temperature

Time

80

20 min

 

Methylation effect

Methylation

dam

dcm

CpG

Cleavage

Cleavage

Cleavage

Cleavage

 

Enzyme activity in EzBuffer

I

II

III

IV

FastCut

50%

100%

100%

75%

100%

 

Product component

- Nco I

- 10X EzBuffer III

- 10X FastCut Buffer

- 6X DNA loading Buffer

- Sterile water

 

Note

Nco I is not affected by damdcm or mammalian CpG methylation. Its recognition sequence includes ATG, and therefore it is possible to express a target protein without additional amino acids after cloning an Nco I-cleaved fragment to the initiation site of an expression vector.

  


 

Figure 1. Unit definition

One unit is defined as the amount of enzyme required to digest 1 µg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 µl. 

Certificates of analysis

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