[Restriction endonucleases] The Buffer Composition of Restriction Endonuclease
2020.01.07 03:33

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(​September 28, 2020 update​​)


· 1X EzBuffer I

10 mM Bis Tris Propane-HCl (pH 7.0 @25 °C), 10 mM MgCl2, 100 μg/ml BSA

 

· 1X EzBuffer II 

10 mM Tris-HCl (pH 7.9 @25 °C), 10 mM MgCl2, 50 mM NaCl, 100 μg/ml BSA

 

· 1X EzBuffer III 

50 mM Tris-HCl (pH 7.9 @25 °C), 10 mM MgCl2, 100 mM NaCl, 100 μg/ml BSA

 

· 1X EzBuffer IV 

20 mM Tris-acetate (pH 7.9 @25 °C), 10 mM magnesium acetate, 50 mM potassium acetate, 100 μg/ml BSA

 

· 1X EzBuffer BamH I 

10 mM Tris-HCl (pH 7.9 @ 25℃), 150 mM NaCl, 10 mM MgCl2, 100 μg/ml BSA

 

· 1X EzBuffer EcoR I 

100 mM Tris-HCl (pH 7.5 @ 25℃)​, 50 mM NaCl, 10 mM MgCl2​, 0.025% Triton X-100

 

· 1X EzBuffer Acc III 

10 mM Tris-HCl (pH 8.5 @25℃), 100 mM NaCl, 10 mM MgCl2

 

· 1X EzBuffer Bal I

50 mM Tris-HCI (pH 8.2 @ 25℃), 5 mM MgCl2

 

· 1X EzBuffer Dpn II

50 mM Bis Tris-HCI (pH 6.0 @ 25℃), 10 mM MgCl2, 100 mM NaCl

 

· 1X EzBuffer Cfr10 I 

10 mM Tris-HCl (pH 8.5 @ 25), 3 mM MgSO4, 100 mM KCl, 0.02% Triton X-100

 

· 1X EzDiluent A

10 mM Tris-HCl (pH 7.4 @25℃), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 μg/ml BSA, 50% glycerol

 

· 1X EzDiluent B

10 mM Tris-HCl (pH 7.4 @25 ℃), 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 500 μg/ml BSA, 50% glycerol

 

· 1X EzDiluent C

10 mM Tris-HCl (pH 7.4 @25 ℃), 250 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.15% Triton X-100, 200 μg/ml BSA, 50% glycerol

 

 

 

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