• Restrction enzyme
  • A :

    Enzynomics has developed FastCut Buffer​ which is compatible for all the Enzynomics restriction enzymes. Therefore, FastCut Buffer​ can be used without hesitation for any double digestion reaction. Moreover, 1 µl of restriction enzyme without dilution give rise to complete digestion within 15 min using FastCut Buffer​.

  • Kit
  • A :

    5’ of the insert DNA should not contain phosphate due to the characteristics of Topoisomerase in TOPcloner™ cloning kit. Therefore, restriction enzyme digested DNA could not use with the TOPcloner™.


  • A :

    Difference is in the components of the TOPcloner™ TA Kit and TOPcloner™ TA core Kit.

    Components are as in the following chart.


     TOPcloner™ TA Kit

     TOPcloner™ TA core Kit

     pTOP TA V2

     pTOP TA V2

     6X TOPcloner™ buffer

     6X TOPcloner™ buffer

     Sterile water

     Sterile water

     2X TOPsimple™ DyeMIX-Tenuto

     

     DH5α Chemically Competent E. coli

     

     M13F, M13R (10 pmol/µl)

     

     Control insert (20 ng/µl)

     

     Control primers (10 pmol/µl)

     



  • A :

    TOPcloner™ cloning kit provides easy and rapid cloning method with the use of topoisomerase.



  • Add.

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  • Tel. +82 - 42 - 719 - 1023
  • Fax. +82 - 42 - 719 - 1024
  • Email. info@enzynomics.com

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